MULTIDISCIPLINARY ANALYSIS OF HIV-1 ELITE

Flow Cytometry to Estimate Leukemia Stem Cells in Primary

The BD LSR Fortessa is equipped with 5 lasers (355, 405, 488, 561,640 nm). It allows detection of up to 18 parameters simultaneously, 16 fluorescent channels plus forward and side scatter. See configuration. For more information about the LSR Fortessa please visit the Becton Dickinson website or contact dirk.pacholsky@igp.uu.se.

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LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b. BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode.

FACS Diva quick The BD LSR Fortessa SORP is a 4-laser flow cytometer capable of detecting up to 18 fluorescence parameters simultaneously with state of the art optics to ensure maximal excitation, and collection of signals.

MULTIDISCIPLINARY ANALYSIS OF HIV-1 ELITE

BD LSR Fortessa Standard Operation Protocol Refill Sheath Container 1. Make sure the flow cytometer is in STANDBY mode.

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BD Fortessa The BD Fortessa cell analyzer provides the flexibility to support measurement of multicolor flow cytometry assays of up to 16 parameters at a time. The FCF is equipped with one BD LSRFortessa™ (Diagnostic Purpose) and a BD LSRFortessa™ X-20 (Research Purpose). Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis. Designed for customization and optimized for reliability, the BD LSRFortessa family of cell analyzers allow you freedom of choice to achieve your research goals–your way. The BD LSR Fortessa™ X-20 cell analyzer delivers high-performance multicolor analysis detecting up to 18 parameters simultaneously with using 5 lasers: UV (355 nm) Violet (405 nm) Blue (488 nm) Y-G (561 nm) Red (640 nm) Fortessa X20 Flow Cytometer Quick guide Operation of the 5 laser Fortessa X20 BD Webinar CS&T System BD Compensation Guide… FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. Fortessa behind right door above control panel) via the silicon tube to the sample needle and pull the syringe plunger.

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Our newest analytical cytometer is the BD Fortessa, based on the venerable LSRII platform but reconfigured to a smaller footprint. This instrument is equipped with 405 nm, 488 nm, 561nm, 635 nm and 785 nm lasers. Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis.

SOP: LSR-Fortessa Instrument Start-up 1. Locate the green power button on the right hand side of the instrument; press to switch on the LSR-Fortessa.
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by pressing the metal button facing the main Fortessa instrument.

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Room 4161.

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